Peer Reviewed Publications

    Zhang, X., Wang, X. X., & Shusta, E. V. (2014). Creation and evaluation of a single‐chain antibody tetramer that targets brain endothelial cells. AIChE Journal. [Abstract]


    Jones, A. R., Stutz, C. C., Zhou, Y., Marks, J. D., & Shusta, E. V. (2014). Identifying blood–brain‐barrier selective single‐chain antibody fragments. Biotechnology journal.  [Abstract]


    Lippmann, E. S., Al-Ahmad, A., Azarin, S. M., Palecek, S. P., & Shusta, E. V. (2014). A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources. Scientific reports, 4.[Abstract]


    Stutz CC, Zhang X, Shusta EV. Combinatorial Approaches for the Identification of Brain Drug Delivery Targets. 2013. Jun 19.  [Abstract]


    Marshall CJ, Agarwal N, Kalia J, Grosskopf VA, McGrath NA, Abbott NL, Raines RT, Shusta EV. Facile Chemical Functionalization of Proteins through Intein-Linked Yeast Display. Bioconjugate Chemistry. 2013. Sep 18;24(9):1634-44.  [Abstract]


    Tillotson BJ, de Larrinoa IF, Skinner CA, Klavas DM, Shusta EV. Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources. . Protein Engineering, Design and Selection. 2013 Feb;26(2):101-12. [Abstract


    Lippmann ES, Al-Ahmad A, Palecek SP, Shusta EV. Modeling the blood-brain barrier using stem cell sources. Fluid Barriers CNS. 2013 Jan 10;10(1):2. [Abstract


    Pavoor T.V., Wheasler J.A., Kamat V., Shusta E.V. An enhanced approach for engineering thermally stable proteins using yeast display. Protein Engineering, Design and Selection, 25 (10): 625-630, 2012. [Abstract


    Agarwal N, Lippmann ES, Shusta EV.  Identification and expression profiling of blood–brain barrier membrane proteins.  J. Neurochem. Nov 6, 2010. [Full Text]  [Abstract


    Cho YK, Shusta EV.  Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.  Protein Engineering, Design and Selection.  May 23, 2010.  [Full Text]  [Abstract]  


    Pavoor TJ,  Cho YK, Shusta EV.  Development of GFP-based biosensors possessing the binding properties of antibodies.  Proc Natl Acad Sci USA.  July 21, 2009. [Full Text]  [Abstract]  


    Pepper LR, Cho YK, Boder ET, Shusta EV.  A decade of yeast surface display technology: Where are we now?.  Comb Chem High Throughput Screen.  May 13, 2009.  [Abstract]


    Agarwal N, Shusta EV.  Multiplex expression cloning of blood–brain barrier membrane proteins.  Proteomics. Jan 29, 2009.  [Abstract]


    Cho YK, Chen I, Wei X, Li L, Shusta EV.  A yeast display immunoprecipitation method for efficient isolation and characterization of antigens. J Immunol Methods.  Feb 28, 2009. [Full Text]  [Abstract]  


    Huang D, Gore PR, Shusta EV.  Increasing yeast secretion of heterologous proteins by regulating expression rates and post-secretory loss.  Biotechnol Bioeng.  Dec 14, 2008.  [Abstract]


    Wentz AE, Shusta EV.  Enhanced Secretion of Heterologous Proteins from Yeast by Overexpression of Ribosomal Subunit RPP0.  Biotechnol Prog.  Sept 5, 2008. [Full Text] [Abstract]


    Jones AR, Shusta EV. Blood-Brain Barrier Transport of Therapeutics via Receptor-Mediation. Pharm Res. Epub July 10, 2007. [Abstract]


    Calabria AR, Shusta EV. A genomic comparison of in vivo and in vitro brain microvascular endothelial cells. J Cereb Blood Flow Metab. Epub June 13, 2007 [Abstract]


    Wang XX, Cho YK, Shusta EV. Mining a yeast library for brain endothelial cell-binding antibodies. Nat Methods. 2007;4:143-145.[Abstract]


    Weidenfeller C, Svendsen CN, Shusta EV. Differentiating embryonic neural progenitor cells induce blood-brain barrier properties. J Neurochem. 2007;101:555-565.[Abstract]


    Wentz AE, Shusta EV. A novel high-throughput screen reveals yeast genes that increase secretion of heterologous proteins. Appl Environ Microbiol. 2007;73:1189-1198.[Abstract]


    Huang D, Shusta EV. A yeast platform for the production of single-chain antibody green fluorescent protein fusions. Appl Environ Microbiol. 2006;72:7748-7759.[Full Text][Abstract]


    Calabria AR, Shusta EV. Blood-brain barrier genomics and proteomics: elucidating phenotype, identifying disease targets, and enabling brain drug delivery. Drug Discov Today. 2006;11:792-799. [Abstract]


    Calabria AR, Weidenfeller C, Jones AR, de Vries HE, Shusta EV. Puromycin-purified rat brain microvascular endothelial cell cultures exhibit improved barrier properties in response to glucocorticoid induction. J Neurochem. 2006;97:922-933. [Abstract]


    Hackel BJ, Huang D, Bubolz JC, Wang XX, Shusta EV. Production of soluble and active transferrin receptor-targeting single-chain antibody using saccharomyces cerevisiae. Pharm Res. 2006;23:790-797. [Abstract]


    Wang XX, Shusta EV. The use of scFv-displaying yeast in mammalian cell surface selections. J Immunol Methods. 2005;304:30-42. [Abstract]


    Huang D, Shusta EV. Secretion and surface display of green fluorescent protein using the yeast saccharomyces cerevisiae. Biotechnol Prog. 2005;21:349-357. [Full Text] [Abstract]


    Shusta EV. Blood-brain barrier genomics, proteomics, and new transporter discovery. NeuroRx. 2005;2:151-161. [Full Text] [Abstract]


    Shusta EV, Li JY, Boado RJ, Pardridge WM. The Ro52/SS-A autoantigen has elevated expression at the brain microvasculature. Neuroreport. 2003;14:1861-1865. [Abstract]


    Shusta EV, Zhu C, Boado RJ, Pardridge WM. Subtractive expression cloning reveals high expression of CD46 at the blood-brain barrier. J Neuropathol Exp Neurol. 2002;61:597-604. [Abstract]


    Shusta EV, Boado RJ, Pardridge WM. Vascular proteomics and subtractive antibody expression cloning. Mol Cell Proteomics. 2002;1:75-82. [Full Text] [Abstract]


    Shusta EV, Boado RJ, Mathern GW, Pardridge WM. Vascular genomics of the human brain. J Cereb Blood Flow Metab. 2002;22:245-252. [Abstract]


    Kieke MC, Sundberg E, Shusta EV, Mariuzza RA, Wittrup KD, Kranz DM. High affinity T cell receptors from yeast display libraries block T cell activation by superantigens. J Mol Biol. 2001;307:1305-1315. [Abstract]


    Shusta EV, Holler PD, Kieke MC, Kranz DM, Wittrup KD. Directed evolution of a stable scaffold for T-cell receptor engineering. Nat Biotechnol. 2000;18:754-759. [Abstract]


    Holler PD, Holman PO, Shusta EV, O'Herrin S, Wittrup KD, Kranz DM. In vitro evolution of a T cell receptor with high affinity for peptide/MHC. Proc Natl Acad Sci U S A. 2000;97:5387-5392. [Full Text] [Abstract]


    Shusta EV, Kieke MC, Parke E, Kranz DM, Wittrup KD. Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency. J Mol Biol. 1999;292:949-956. [Abstract]


    Kieke MC, Shusta EV, Boder ET, Teyton L, Wittrup KD, Kranz DM. Selection of functional T cell receptor mutants from a yeast surface-display library. Proc Natl Acad Sci U S A. 1999;96:5651-5656. [Full Text] [Abstract]


    Shusta EV, VanAntwerp J, Wittrup KD. Biosynthetic polypeptide libraries. Curr Opin Biotechnol. 1999;10:117-122. [Abstract]


    Shusta EV, Raines RT, Pluckthun A, Wittrup KD. Increasing the secretory capacity of saccharomyces cerevisiae for production of single-chain antibody fragments. Nat Biotechnol. 1998;16:773-777. [Abstract]