Dagang Huang

Ph.D. 2007

Research Project

My current research is to understand the impediments to protein secretion in yeast Saccharomyces cerevisiae. We created the first green fluorescent protein (GFP) secretion model system, which provides a powerful tool to study the real-time protein trafficking in the secretory pathway. Using this system, we studied secretion of therapeutic and potential therapeutic single-chain antibody variable fragments (scFv) tagged with GFP. The dynamics of protein synthesis, secretion, and degradation was also studied. The findings explained the temperature effect on secretion level and the development of intracellular accumulation of the expressed protein and point out the future directions for alleviating the bottlenecks to protein secretion.

Previous Education

B.S. in Chemical Engineering
Tsinghua University, Beijing China

M.S. in Chemical Engineering
Tsinghua University, Beijing China

Hometown

Qidong, Jiangsu Province, China

Publications

  1. Huang D, Gore PR,Shusta EV. Study on foreign secretion dynamics in yeast Saccharomyces cerevisiae to increase protein production yield. In preparation
  2. Huang D, Shusta EV. A Yeast Platform for the Production of Single-Chain Antibody-Green Fluorescent Protein Fusions. Applied and Environ Microbiology, 72(12): 7748-7759 2006
  3. Hackel BJ, Huang D,Bubolz JC, Wang XX, Shusta EV. Production of Soluble and Active Transferrin Receptor-Targeting Single-Chain Antibody using Saccharomyces cerevisiae. Pharmaceutical Research 23(4): 790-797 2006
  4. Huang D, Shusta EV. Secretion and surface display of green fluorescent protein using the yeast Saccharomyces cerevisiae. Biotechnology Progress 21 (2): 349-357 2005
  5. Huang D, Han MH,Wang JF, Jin Y. Catalytic decomposition process of cumene hydroperoxide using sulfonic resins as catalyst. Chemical Engineering Journal, 88 (1-3): 215-223 2002
  6. Huang D, Yu SR,  Han MH etc., Decomposition of Cumene Hydroperoxide Catalyzed by Resins I. Catalysts, Chinese Journal of Process Engineering, 1(2): 97-201 2001
  7. Huang D, Han MH, Wang JF, and Jin Y. Decomposition of Cumene Hydroperoxide Catalyzed by Resin II. Reaction Process, Chinese Journal of Process Engineering, 1(3): 304-308 2001
  8. Han MH, Huang D, Lu Y, Cao G. Decomposition of Cumene Hydroperoxide Catalyzed by Resins in a Three-Phase Circulating Fluidized Bed Reactor I. Reaction Process, Petrochemical Technology, 30(6): 433-436 2001
  9. Han MH, Huang D, Wang JF, and Jin Y. Decomposition of cumene Hydroperoxide Catalyzed by Resins in a Three-Phase Circulating Fluidized Bed Reactor II. Kinetics and Mass Transfer, Petrochemical Technology, 30(7): 531-533 2001